l2 murine fibroblast cell line  (ATCC)

Journal:

Article Title: Inhibition of ROCK activity allows InlF-mediated invasion and increased virulence of Listeria monocytogenes

doi: 10.1111/j.1365-2958.2008.06188.x

Figure Lengend Snippet: Cell type specific effects of ROCK activity inhibition on L. monocytogenes infection. Monolayers of cell lines were treated with 10 μM Y27632 or DMSO (control) for 30 min prior to washing of monolayers and infection with wild-type L. monocytogenes 10403S. Cell-associated (total) and intracellular bacteria were quantified by gentamicin protection assay as described in Experimental Procedures. Data presented represents the means ±s.d. fold change in CFU upon Y27632 treatment relative to control samples for one of three experiments performed in triplicate with similar results. * p <0.01; bold type indicates changes in CFU greater than 2-fold with p< 0.05.

Article Snippet: The human-derived epithelial cell lines HeLa (ATCC CCL-2) and HEp-2 (ATCC CCL-23), the murine-derived epithelial cell line 1308.1, the murine-derived fibroblast cell lines L2 and L929, the murine-derived macrophage cell lines RAW 264.7 and RAW 309 Cr.1, and the human-derived macrophage cell line U937 (ATCC CRL-1593.2) were cultured in RPMI 1640 medium (Mediatech, Herndon, VA) supplemented with 10% FBS (HyClone, Logan, UT), 55 μM 2-β-mercaptoethanol, 1 mM sodium pyruvate, and 2 mM glutamine.

Techniques: Activity Assay, Inhibition, Infection, Control, Bacteria